细胞名称

CRFK猫肾细胞

描述

此细胞不含牛病毒性痢疾(BVD)病毒。

动物种别

猫

性别

雌

组织来源

肾，皮层；正常

形态

上皮细胞

培养基和添加剂

RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

REFERENCE

Crandell RA , et al. Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176-185, 1973. PubMed: 4130570 Fabricant CG , Rich LJ . Microbial studies of feline urolithiasis. J. Am. Vet. Med. Assoc. 158: 976-980, 1971. PubMed: 4930310 Lee KM , et al. Use of an established cat cell line for investigation and quantitation of feline oncornaviruses. J. Natl. Cancer Inst. 49: 55-60, 1972. PubMed: 4338781 Loffler S , et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321